Protein_Domain
CBD

Part:BBa_K1830003:Design

Designed by: Mathieu Husser   Group: iGEM15_Concordia   (2015-08-06)

CBD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

CCG to CCA mutation to remove AgeI cut site

GAA to GAG mutation to remove EcoRI cut site

Source

Clostridium Thermocellum CipA protein, GenBank accession number: L08665.1 (nucleotide) / Q06851 (protein)

References

Gerngross, U. T., Romaniec, M. P. M., Kobayashi, T., Huskisson, N. S., & Demain, A. L. (1993). Sequencing of a Clostridium thermocellum gene (cipA) encoding the cellulosomal SL‐protein reveals an unusual degree of internal homology. Molecular microbiology, 8(2), 325-334.

Wieczorek, A. S., & Martin, V. J. (2010). Engineering the cell surface display of cohesins for assembly of cellulosome-inspired enzyme complexes on Lactococcus lactis. Microbial cell factories, 9(1), 69.